Research | Open | Published:
Effects of capture and captivity on plasma corticosterone and metabolite levels in breeding Eurasian Tree Sparrows
Avian Researchvolume 10, Article number: 16 (2019)
Bringing free-living animals into captivity subjects them to the stress of both capture and captivity, leading to the alteration of normal physiological processes and behaviors through activation of the hypothalamic–pituitary–adrenal axis. In free-living birds, although elevated plasma corticosterone (CORT) is an important adaptation regulating physiological and behavioral responses during the process of capture and captivity stress, little information is currently available on the effects of such stress on plasma metabolite levels.
We examined the effects of immediate capture and 24-h captivity on body mass, body condition, plasma CORT, and metabolite levels including glucose (Glu), triglyceride (TG), total cholesterol (TC), uric acid (UA), in breeding Eurasian Tree Sparrows (Passer montanus).
CORT and Glu levels were increased significantly by the stress of capture, whereas TC and UA levels decreased. Body mass, body condition declined notably after 24 h in captivity, but CORT, Glu, and UA levels increased. Furthermore, male sparrows had lower TG levels after both capture and captivity than those of females. The relationships between plasma CORT and metabolite levels varied between sexes.
Our results revealed that the metabolic status of Eurasian Tree Sparrows could be dramatically altered by capture and captivity. Monitoring the dynamic effects of both capture and captivity on plasma CORT, metabolite levels in a free-living bird contributes to a better understanding of the stress-induced pathways involved in sex-dependent energy mobilization.
Bringing free-living animals into captivity is frequently required for conservation, and for research in animal ecology, environmental physiology, and conservation biology (Dickens et al. 2010; Mason 2010; Mason et al. 2013; Dickens and Bentley 2014). This process subjects animals to both the acute stress of immediate capture and the chronic stress of captivity, resulting in the alteration of normal physiology and behavior (Dickens et al. 2010; Angelier et al. 2016). In vertebrates, capture stress may acutely activate the hypothalamic–pituitary–adrenal (HPA) axis to increase glucocorticoid (GC) levels that can enhance survival by regulating behavioral and physiological responses (Romero and Wingfield 1999; Sapolsky et al. 2000; Wingfield and Kitaysky 2002). However, captivity stress may chronically increase GC levels, which may reduce fitness through a suite of deleterious effects such as suppressing parental behavior, compromising reproductive success and promoting metabolic disorders (Dallman 1993; Wingfield et al. 1995; Vegiopoulos and Herzig 2007). Some previous studies have demonstrated the effects of capture and captivity stress on the HPA axis and behavioral responses of free-living animals (Rich and Romero 2005; Cyr et al. 2007, 2009; Dickens and Romero 2009; Dickens et al. 2009; Lattin et al. 2012; Fischer et al. 2018). To date, however, there has been little research on the dynamic changes of both plasma GCs and metabolites during the first hours, or days, free-living animals have spent in captivity (Angelier et al. 2016).
In mammals, stress-induced GCs promote hydrolysis of triglycerides (TG) stored in adipocytes except inducing an increase in circulating glucose (Glu) levels through hepatic gluconeogenesis and maintain hyperglycemia by suppressing the uptake of Glu into peripheral tissues (Grégoire et al. 1991; Yamada et al. 1993). Although increasing GC levels is a general adaptive characteristic used to mobilize energy stores in a variety of metabolic pathways (i.e., increased energy expenditure), the regulatory mechanisms and physiological consequences of capture stress and captivity stress differ (Sapolsky et al. 2000). Compared to mammals, birds can maintain higher, better-controlled, plasma Glu levels (Braun and Sweazea 2008; Li 2017). Although an increase in corticosterone (CORT, the main GC in birds) in response to acute stress is ubiquitous in free-living birds, alteration of plasma Glu levels is not universal. For example, plasma Glu was found to increase in response to capture stress in Abert’s Towhees (Melozone aberti) and King Penguin (Aptenodytes patagonicus) chicks (Corbel et al. 2010; Davies et al. 2013), but not in Curve-billed Thrashers (Toxostoma curvirostre) (Fokidis et al. 2011). Moreover, plasma Glu response to capture stress varies with life-history stages in Rufous-winged Sparrows (Peucaea carpalis), i.e. not change in breeding stage, and decreased in molting and non-breeding stages (Deviche et al. 2016a). The reason for this variability in plasma Glu regulation in free-living birds remains largely unknown. In birds, plasma TG has been widely used as an indicator of body mass or condition (Guglielmo et al. 2005). A rapid decrease in the TG of free-living birds can be detected as soon as 20 min after capture (Guglielmo et al. 2002). The confinement, unfamiliar and unnatural conditions experienced by free-living animals in captivity can lead to the secretion of plasma GC and metabolites (e.g. Glu and TG), resulting in metabolic disorders, energetic stress and reduced fitness (Morgan and Tromborg 2007; Dickens et al. 2009, 2010; Mason 2010; Fokidis et al. 2011).
Uric acid (UA) is a product of protein degradation (Costantini 2008) and is also a potent antioxidant (Stinefelt et al. 2005; Cohen et al. 2007; Braun and Sweazea 2008). Capture stress can lead to decreased plasma UA in some bird species, which is believed to function as an antioxidant defense against increased free radicals during oxidative stress (Cohen et al. 2007; Davies et al. 2013). However, chronic stress of captivity may generally raise UA levels, which is thought to promote gluconeogenesis by increasing the use of amino acids (Klasing 1998). Cholesterol is an essential constituent of cell membranes modulating their fluidity and is also a precursor of steroid hormones. Hypocholesterolemia may be associated with low serum antioxidant reserves and may increase susceptibility to oxidative stress (Muldoon et al. 1996). It has, therefore, been hypothesized that cholesterol may act as an antioxidant (Schroepfer 2000). Even though oxidative stress typically triggers the stress response process (Costantini 2008), few studies have linked it to the modulation of stress response mediated by GC release.
Male and female birds exhibit sex-specific physiology associated with their differentiated reproductive functions, e.g., development of the testis and spermatogenesis in males and development of the ovary and oogenesis in females. In free-living birds, the effects of both capture and captivity stress on sex-specific CORT responses have been well documented (Dickens et al. 2010; Breuner 2011). However, the influences of capture and captivity stress on sex-specific metabolites have received little attention.
To determine the dynamic effects of both capture stress and subsequent captivity stress on plasma CORT and metabolites, we studied changes in plasma CORT, metabolite levels, including Glu, TG, total cholesterol (TC), and UA, of breeding Eurasian Tree Sparrows (Passer montanus) that were subject to both the stress of capture in the field and a subsequent 24-h period in captivity. The Eurasian Tree Sparrow is a typical human commensal species that distributes throughout the Eurasian continent (Summers-Smith 2014). In recent years, the reproductive biology and endocrinology of the Eurasian Tree Sparrow has been relatively well studied, e.g. their adrenocortical responses to capture stress during the breeding season in relation to body condition, testosterone (T), corticosteroid-binding globulin (CBG; Li et al. 2008, 2011, 2012, 2016; Zhao et al. 2017a, b). We hypothesized that (1) Glu would increase, and TG decrease in breeding Eurasian Tree Sparrows, concomitantly with increased CORT levels in response to both capture and captivity stress; (2) plasma UA would decrease and UA increase after capture and 24-h captivity stress; (3) male and female sparrows would differ in both baseline and stress-induced (capture and captivity) metabolite levels.
Animals and study sites
A total of 22 Eurasian Tree Sparrows (12 males and 10 females) were captured opportunistically with mist nets between 06:00 and 10:00 at the Shijiazhuang Academy of Agriculture and Forestry Sciences (38°06.71′N, 114°31.49′E, elevation: 73 m), and at the old (38°01.83′N, 114°31.50′E, elevation: 75 m), and new (37°59.88′N, 114°31.18′E, elevation: 72 m) campuses of Hebei Normal University, Hebei Province, China. All capture sites were situated in similar habitat near farmlands and buildings. All sparrows were captured during the breeding season: May 15–June 9, 2012‒2013.
Experiment design of capture and captivity stress
All wild-caught sparrows were subject to a standardized capture-handling-restraint stress protocol (Wingfield et al. 1992; Li et al. 2008, 2011) within 1 h after capture in the field, followed by 24 h of captivity stress in cages. After capture and sampling in the field, each bird was individually housed in cages (40 cm × 30 cm × 30 cm) and provided with foxtail millet (Setaria italica) mixed with mealworms and water ad libitum, and transferred to a laboratory at Hebei Normal University. To reduce confounding variables the temperature and photoperiod in the laboratory were kept as close as possible to those in the birds’ natural environment (temperature: 17‒29 °C; photoperiod: 14.2‒14.9 h).
Blood sampling and morphological measurements
In the field, baseline levels of plasma CORT and metabolites were sampled within 3 min of capture, and capture stress-induced levels were sampled 30 and 60 min post-capture. Briefly, a wing vein was punctured with a 26 gauge needle, and approximately 80 μL of blood was collected into heparinized microhematocrit capillary tubes. Birds were then placed in an opaque cloth bag, and additional blood samples were collected from them after another 30 and 60 min post-capture. In the laboratory, captivity stress-induced levels of plasma CORT and metabolites were sampled at the end of the 24 h period of captivity within 3 min of extracting birds from their cages. All blood samples were stored on ice for 3‒4 h until they could be centrifuged in the laboratory at 855 × g for 10 min. Plasma samples were stored at − 20 °C until assayed.
After blood sampling was completed, each bird was weighed to ± 0.1 g, and its wing length, tarsus length, and the width and height of the cloacal protuberance, measured to ± 1 mm. Following Zhao et al. (2017b), we used the ratio of an individual bird’s body mass (g) to wing length (mm)3 as an index of its body condition. Birds were sexed by presence or absence of a brood patch (only females have a brood patch), and by cloacal protuberance size and wing length (males have significantly larger cloacal protuberances and longer wings than females).
Assays of plasma CORT and metabolites
Plasma CORT levels were measured using enzyme immunoassay kits according to the manufacturer’s instructions (Cat No. ADI-901-097, Enzo Life Sciences) with minor modifications (Li et al. 2011). Briefly, 7 μL of plasma was diluted with 42 μL of 2% steroid displacement buffer. After 15 min, 230 μL of assay buffer was added to each sample, vortexed and a 100 μL aliquot of this mixture placed in an individual well. Standard curves with six dilutions ranging from 12,500 to 4 pg of CORT were obtained from each sample. An extra standard of 100 pg of CORT was run in each plate to assess inter-assay variation. All samples were run in duplicate. Intra- and inter-assay variation were 4.5% and 7.9%, respectively. Assay sensitivity was 0.9 ng/mL.
Plasma metabolites (Glu, TG, TC, UA) were measured in 25 μL plasma samples diluted with dH2O (1:39) using an automatic biochemical analyzer (Mindray BS-180) with commercially available kits (Mindray Corp., Shenzhen, China). All samples were run in duplicate. Intra- and inter-assay variation were 3.1% and 8.2% (Glu), 9.6% and 10.1% (TG), 7.9% and 9.2% (TC), 8.4% and 11.6% (UA), respectively. Assay sensitivity was 0.3 mmol/L (Glu), 0.03 mmol/L (TG), 0.04 mmol/L (TC), 14.2 μmol/L (UA).
To identify the factors that affected body mass, plasma CORT, metabolites, and enzyme activity, a generalized linear mixed model (GLMM) was used to assess the significance of the fixed effects of repeated-measures sampling (time), sex, and the interaction between time and sex, and the random effects of sampling year and site. We used SPSS 24.0 software to fit GLMMs and estimate F statistics, degrees of freedom and p-values. The statistical significance of differences between pairs of means was assessed using Bonferroni-adjusted post hoc tests based on the means estimated by the GLMMs or using independent t-tests. Spearman correlations were used to investigate relationships between baseline (within 3 min of capture) or stress-induced CORT levels (30 and 60 min post-capture and after 24 h in captivity) and metabolites. p values < 0.05 were considered significant.
Effects of capture and 24-h captivity stress on body mass and plasma CORT
Both body mass and CORT levels varied markedly with sampling time, independent of sex, and the interaction between time and sex (Table 1). Body condition index varied with sample time and sex, independently of the interaction between time and sex (Table 1). Post hoc results showed that both body mass and body condition decreased significantly during the 24-h period birds were held in captivity (Fig. 1a, b), and that female sparrows were in better condition than males (Fig. 1b). Both the stress of capture and captivity caused CORT levels to increase significantly relative to baseline levels (Fig. 1c; Additional file 1: Table S1). There was no significant difference between CORT levels measured 30 min after capture and those measured after 24 h in captivity (Fig. 1c; Additional file 1: Table S1).
Effects of capture and 24-h captivity on plasma Glu, TC, TG, and UA
Plasma Glu, TC, and UA levels varied significantly with sampling time, independent of sex, and the interaction between time and sex (Table 1). Post hoc test results revealed that Glu levels measured 30 and 60 min after capture were significantly higher than baseline levels (Fig. 2a; Additional file 1: Table S1). TC levels decreased notably at 60 min after capture relative to baseline levels (Fig. 2c; Additional file 1: Table S1). UA levels were significantly lower at 30 min and 60 min post-capture than baseline levels (Fig. 2b; Additional file 1: Table S1). Glu levels measured after 24 h in captivity were significantly higher than both baseline levels and those measured 60 min post-capture, and UA levels were also markedly higher after 24 h in captivity than both the baseline level and those measured 30 min and 60 min post-capture (Fig. 2a, b; Additional file 1: Table S1). However, TC levels after 24 h in captivity did not differ from those of baseline and stress-induced levels after capture.
Plasma TG levels did not vary with sampling time but varied significantly with sex, independent of the interaction between time and sex (Table 1). Male sparrows had significantly lower TG levels than females (Fig. 2d).
Correlations between CORT and metabolites
Baseline plasma CORT levels did not correlate with baseline levels of Glu, TG, TC, and UA in both male and female sparrows (Table 2). Stress-induced CORT levels at 30 min and 60 min after capture were not correlated with any variables except for a positive relationship between stress-induced CORT and TG levels 30 min post-capture in females (Table 2; Fig. 3a). Captivity stress-induced CORT levels in females were negatively correlated Glu, TG, and positively correlated with UA levels (Table 2; Fig. 3b, c, d). Furthermore, although baseline TG levels were neither correlated with body mass (male: r = − 0.291, p = 0.385; female: r = − 0.587, p = 0.126) nor body condition (male: r = − 0.064, p = 0. 853; female: r = − 0.214, p = 0.610), baseline TC levels were positively correlated with UA levels in both sexes (male: r = 0.682, p = 0.012; female: r = 0.905, p = 0.039).
In Eurasian Tree Sparrows, although both the stress of capture and 24 h in captivity elevated plasma CORT and Glu levels, they had different effects on TC and UA levels. Furthermore, TG levels of males and females differed. Free-living Eurasian tree sparrows were caught and sampled on the day of capture and re-sampled the day after bringing them to the laboratory. Therefore, the observed changes in plasma CORT and metabolites may have been due to the combined effects of initial capture stress followed by 24 h in captivity.
Effects of capture stress
Capture stress protocol has been widely used in evaluating acute stress on the physiological changes in free-living animals, which includes not only an unpredictable capture-handling stimulus but also a stimulus of short-term fasting (Wingfield et al. 1992). In the present study, free-living Eurasian tree sparrows showed an increase in plasma CORT levels 30 min after capture. Such acute responses are generally thought to allow animals to cope physiologically and behaviorally with perturbations in the environment (Romero 2002). Meanwhile, plasma Glu levels followed a similar pattern to CORT. This result is consistent with the increase in Glu observed in king penguin chicks and Abert’s towhees (Corbel et al. 2010; Davies et al. 2013), and pre-breeding rufous-winged sparrows (Deviche et al. 2016a). During capture stress, mobilizing carbohydrate stores as the principal energy source has been considered the most efficient way to transition to the emergency life history stage (Wingfield et al. 1998; Landys et al. 2006). Increased stress-induced CORT is believed to be essential to maintain hyperglycemia by suppressing the Glu uptake of peripheral tissues (Landys et al. 2004) and promoting hepatic gluconeogenesis (Jenni-Eiermann et al. 2002). However, recent avian studies demonstrated that increased plasma CORT is not necessarily associated with hyperglycemia (Fokidis et al. 2011; Deviche et al. 2014, 2016a, b). Therefore, the underlying mechanism of plasma CORT in regulating Glu levels in free-living animals may differ from previous findings.
TG levels in Eurasian tree sparrows did not change in response to capture stress and were not correlated with body mass or body condition. Consistent with our results, capture stress did not reduce the TG plasma levels of urban curve-billed thrashers (Fokidis et al. 2011). However, capture stress has been found to decrease TG levels in both free-living desert curve-billed thrashers and western sandpipers (Calidris mauri), which suggests TG catabolism and inhibition of lipogenesis (Guglielmo et al. 2002; Fokidis et al. 2011). It was demonstrated that the changes of TG levels during capture stress might be dependent on body condition, i.e., those individuals with better body condition do not necessarily decrease their plasma TG levels (Fokidis et al. 2011). Therefore, the effect of capture stress on TG levels may depend on an individuals’ energy status. Further research is required to determine the correlation between plasma TG and energy status in free-living birds, and the modulation of lipid metabolism in response to capture stress.
Consistent with the results of studies on some other species (Cohen et al. 2007; Davies et al. 2013; Deviche et al. 2014, 2016a), the UA levels of Eurasian tree sparrows decreased 30 min and 60 min after capture (Fig. 2b) suggesting an inhibitory effect of acute stress on plasma UA. UA, a non-enzymatic antioxidant, may act as the first line of defense against the proliferation of free radicals (Cohen et al. 2007). Increasing tissue uptake of UA during capture stress may, therefore, be especially beneficial with regard to improving antioxidant capacity (Cohen et al. 2007; Strazzullo and Puig 2007; Sautin and Johnson 2008; Deviche et al. 2014).
In birds, plasma cholesterol is obtained from the diet or hepatic production (Hazelwood 1972), and may preferentially be directed toward steroidogenesis (e.g., CORT, T, and estradiol) and spermatogenesis (Orgebin-Crist and Tichenor 1973; Tift et al. 2011). To the best of our knowledge, our results provide the first evidence of reduced TC levels in response to capture stress, and of a positive correlation between baseline TC levels and UA levels in a free-living animal. These findings indicate that the regulation of cholesterol metabolism may be associated with enhanced catabolism and oxidative stress response induced by the stress of capture. Although this observation is consistent with reduced TC levels in humans suffering from depression (Partonen et al. 1999), the acute stress of immobilization did not affect the TC levels of experimental rats (Hershock and Vogel 1989). Why the TC levels of Eurasian tree sparrows decreased in conjunction with reduced UA levels and how this is physiologically related to the oxidative stress response remains unclear. Further research is therefore required to investigate the change in TC levels in response to capture stress in free-living animals.
Effects of 24-h captivity stress
Eurasian Tree Sparrows experienced prolonged effects of the initial capture and restraint stress of captivity and underwent significant weight loss in captivity despite water and food were provided ad libitum. The underlying causes of weight loss may directly result from 24 h of captivity stress that not only subjected birds to the stress of confinement but also deprived them of their natural diet, which may have caused them to fast or reduce their food intake. Although we did not measure food consumption or energy expenditure, we found that plasma CORT levels increased after 24 h in captivity. This result is consistent with the reduced weight and elevated baseline CORT observed in captive, wild-caught Chukar (Alectoris chukar) (Dickens et al. 2009), increased CORT levels and reduced body weight after 4‒10 h of fasting in the Zebra Finch (Taeniopygia guttata) (Lynn et al. 2010), and in Rock Pigeons (Columbia livia) after 24 h in captivity (Angelier et al. 2016). Reduced body weight is a hallmark of the elevated baseline CORT (Sapolsky et al. 2000) secreted in response to the chronic stress associated with captivity, e.g., confinement, altered light conditions and diet (Morgan and Tromborg 2007).
GCs are known to inhibit TG synthesis and promote the availability of lipid energy from adipose tissue stores (Dallman 1993; Bentley 1998; Landys et al. 2006), thus providing substrates for continued gluconeogenesis (Grégoire et al. 1991; Landys et al. 2004). Previous studies have shown increased plasma UA as a result of protein degradation after flight or exercise in some avian species (Shmueli et al. 2000; Jenni-Eiermann et al. 2002; Tsahar et al. 2006). In addition, exogenous GCs have also been found to induce proteolysis and muscle atrophy in several species (Landys et al. 2006), which is critical for replenishing amino acid substrates for gluconeogenesis. Along with elevated CORT, increased Glu and UA levels in conjunction with reduced body weight, leads us to speculate that Eurasian Tree Sparrows relied heavily on gluconeogenesis and protein catabolism, during the 24 h they were in captivity. Given that 24 h of captivity did not induce the changes of TC and TG levels compared with their baseline levels in the field, whether the lipids catabolism was enhanced in response to captivity stress remains unclear.
Sexual differences in stress responses
There were no significant differences between the sexes with respect to changes in body mass, plasma CORT, Glu, TC, and UA levels following both capture and captivity. Furthermore, plasma CORT was not correlated with Glu and UA levels following capture stress. These results are consistent with those of previous studies that also found no sexual differences in acute CORT response to capture stress in this species during the breeding season (Li et al. 2008, 2011, 2016). This may reflect the fact that both male and female Eurasian tree sparrows incubate eggs and feed nestlings (Summers-Smith 2014). However, sex-specific relationships between plasma CORT and metabolites after capture and 24 h in captivity (Table 2; Fig. 3) suggest condition-dependent variations between sexes, e.g., insufficient glucose, and thus enhanced protein degradation in the females, but not in male sparrows. The underlying cause of this sex-specific correlation between stress-induced CORT and, Glu, and UA levels remains to be further determined.
Our results show that female sparrows were not only in better condition but also had higher plasma TG levels than males. Consistent with our results, male Canada Geese (Branta canadensis) during the spring post-migratory phase also had significantly lower serum TG than females (Mori and George 1978). We further found that stress-induced (30 min post-capture) CORT levels in female sparrows were positively correlated with TG levels whereas this correlation became negative after 24 h in captivity. This trend was not observed in male sparrows. Male sparrows have significantly higher baseline testosterone (T), which is thought to be a mediator of catabolism, than females during breeding (Li et al. 2012). It remains unclear if the higher TG levels of females can be explained by their better body condition when captured, and if such sex-specific differences are due to an interaction between energy metabolism and reproductive physiology.
It should be pointed out that our experimental design does not permit us to determine whether or to what extent the initial capture stress contributed to the endocrine, and metabolic differences observed in the 24-h captivity. It is worthy of mentioning that, initial capture stress-induced elevated plasma Glu levels in Rufous-winged Sparrows, which persisted until the following day when they were re-capture, but plasma CORT and UA levels returned to initial levels (Deviche et al. 2016b). As we mentioned above, the elevated Glu in Eurasian Tree Sparrows observed here may have attributed to the combined effects of initial capture stress followed by 24 h in captivity. How do initial capture stress influence on the plasma Glu and other metabolites on the following day in free-living Eurasian Tree Sparrows, remains to be further investigated.
In the present study, we identified that while the plasma CORT and Glu levels of free-living Eurasian Tree Sparrows increased significantly in response to capture stress, UA and TC levels decreased markedly. These results suggest that wild animals can rapidly regulate their plasma CORT and metabolite levels when subject to acute stress. Furthermore, sparrows subject to 24-h captivity had decreased body mass but increased plasma CORT, Glu, and UA levels. Male sparrows had lower TG after capture and captivity than females, which indicates that there are sex-specific differences in the physiology of this species. Overall, our results show that the stress of capture and captivity can have different effects on plasma CORT, metabolite levels in a passerine bird, which contributes to better understanding of the stress-induced pathways involved in sex-dependent energy mobilization. Our findings can also be applicable to illustrate the physiological alterations of free-living birds, especially endangered specie when bringing them into captivity during the process of ex situ conservation.
Availability of data and materials
The datasets used and analyzed during the current study are available from the corresponding author on reasonable request.
Angelier F, Parenteau C, Trouvé C, Angelier N. Does the stress response predict the ability of wild birds to adjust to short-term captivity? A study of the rock pigeon (Columbia livia). R Soc Open Sci. 2016;3:160840.
Bentley PJ. Hormones that influence intermediary metabolism. In: Bentley PJ, editor. Comparative vertebrate endocrinology. Cambridge: Cambridge University Press; 1998. p. 230–8.
Braun EJ, Sweazea KL. Glucose regulation in birds. Comp Biochem Physiol B. 2008;151:1–9.
Breuner CW. Stress and reproduction in birds. In: Norris DO, Lopez KH, editors. Hormones and reproduction of vertebrates. London: Academic Press; 2011. p. 129–51.
Cohen A, Klasing K, Ricklefs R. Measuring circulating antioxidants in wild birds. Comp Biochem Physiol B. 2007;147:110–21.
Corbel H, Geiger S, Groscolas R. Preparing to fledge: the adrenocortical and metabolic responses to stress in king penguin chicks. Funct Ecol. 2010;24:82–92.
Costantini D. Oxidative stress in ecology and evolution: lessons from avian studies. Ecol Lett. 2008;11:1238–51.
Cyr NE, Dickens MJ, Romero LM. Heart rate and heart-rate variability responses to acute and chronic stress in a wild-caught passerine bird. Physiol Biochem Zool. 2009;82:332–44.
Cyr NE, Earle K, Tam C, Romero LM. The effect of chronic psychological stress on corticosterone, plasma metabolites, and immune responsiveness in European starlings. Gen Comp Endocrinol. 2007;154:59–66.
Dallman MF. Stress update: adaptation of the hypothalamic-pituitary-adrenal axis to chronic stress. Trends Endocrinol Met. 1993;4:62–9.
Davies S, Rodriguez NS, Sweazea KL, Deviche P. The effect of acute stress and long-term corticosteroid administration on plasma metabolites in an urban and desert songbird. Physiol Biochem Zool. 2013;86:47–60.
Deviche P, Beouche-Helias B, Davies S, Gao S, Lane S, Valle S. Regulation of plasma testosterone, corticosterone, and metabolites in response to stress, reproductive stage, and social challenges in a desert male songbird. Gen Comp Endocrinol. 2014;203:120–31.
Deviche P, Valle S, Gao S, Davies S, Bittner S, Carpentier E. The seasonal glucocorticoid response of male Rufous-winged Sparrows to acute stress correlates with changes in plasma uric acid, but neither glucose nor testosterone. Gen Comp Endocrinol. 2016a;235:78–88.
Deviche P, Bittner S, Davies S, Valle S, Gao S, Carpentier E. Endocrine, metabolic, and behavioral effects of and recovery from acute stress in a free-ranging bird. Gen Comp Endocrinol. 2016b;234:95–102.
Dickens MJ, Bentley GE. Stress, captivity, and reproduction in a wild bird species. Horm Behav. 2014;66:685–93.
Dickens MJ, Delehanty DJ, Romero LM. Stress: an inevitable component of animal translocation. Biol Conserv. 2010;143:1329–41.
Dickens MJ, Earle KA, Romero LM. Initial transference of wild birds to captivity alters stress physiology. Gen Comp Endocrinol. 2009;160:76–83.
Dickens MJ, Romero LM. Wild European Starlings (Sturnus vulgaris) adjust to captivity with sustained sympathetic nervous system drive and a reduced fight-or-flight response. Physiol Biochem Zool. 2009;82:603–10.
Fischer CP, Wright-Lichter J, Romero LM. Chronic stress and the introduction to captivity: how wild house sparrows (Passer domesticus) adjust to laboratory conditions. Gen Comp Endocrinol. 2018;259:85–92.
Fokidis HB, Hurley L, Rogowski C, Sweazea K, Deviche P. Effects of captivity and body condition on plasma corticosterone, locomotor behavior, and plasma metabolites in curve-billed thrashers. Physiol Biochem Zool. 2011;84:595–606.
Grégoire F, Genart C, Hauser N, Remacle C. Glucocorticoids induce a drastic inhibition of proliferation and stimulate differentiation of adult rat fat cell precursors. Exp Cell Res. 1991;196:270–8.
Guglielmo CG, Cerasale DJ, Eldermire C. A field validation of plasma metabolite profiling to assess refueling performance of migratory birds. Physiol Biochem Zool. 2005;78:116–25.
Guglielmo CG, O’Hara PD, Williams TD. Extrinsic and intrinsic sources of variation in plasma lipid metabolites of free-living western sandpipers (Calidris mauri). Auk. 2002;119:437–45.
Hazelwood RL. The intermediary metabolism of birds. In: Farner DS, King JR, editors. Avian biology. New York: Academic Press; 1972. p. 472–526.
Hershock D, Vogel WH. The effects of immobilization stress on serum triglycerides, nonesterified fatty acids, and total cholesterol in male rats after dietary modifications. Life Sci. 1989;45:157–65.
Jenni-Eiermann S, Jenni L, Kvist A, Lindström Å, Piersma T, Visser GH. Fuel use and metabolic response to endurance exercise: a wind tunnel study of a long-distance migrant shorebird. J Exp Biol. 2002;205:2453–60.
Klasing KC. Metabolism and storage of triglycerides. In: Klasing KC, editor. Comparative avian nutrition. New York: Centre for Agriculture and Bioscience International; 1998. p. 182–94.
Landys MM, Ramenofsky M, Guglielmo CG, Wingfield JC. The low-affinity glucocorticoid receptor regulates feeding and lipid breakdown in the migratory Gambel’s white-crowned sparrow Zonotrichia leucophrys gambelii. J Exp Biol. 2004;207:143–54.
Landys MM, Ramenofsky M, Wingfield JC. Actions of glucocorticoids at a seasonal baseline as compared to stress-related levels in the regulation of periodic life processes. Gen Comp Endocrinol. 2006;148:132–49.
Lattin CR, Bauer CM, de Bruijn R, Romero LM. Hypothalamus-pituitary-adrenal axis activity and the subsequent response to chronic stress differ depending upon life history stage. Gen Comp Endocrinol. 2012;178:494–501.
Li D. Birds as pathology-free models of type II diabetes. Austin Endocrinol Diabetes Case Rep. 2017;2:1007.
Li D, Wang G, Wingfield JC, Zhang Z, Ding C, Lei F. Seasonal changes in adrenocortical responses to acute stress in Eurasian tree sparrow (Passer montanus) on the Tibetan Plateau: comparison with house sparrow (P. domesticus) in North America and with the migratory P. domesticus in Qinghai Province. Gen Comp Endocrinol. 2008;158:47–53.
Li D, Wu J, Zhang X, Ma X, Wingfield JC, Lei F, Wang G, Wu Y. Comparison of adrenocortical responses to acute stress in lowland and highland Eurasian tree sparrows (Passer montanus): similar patterns during the breeding, but different during the prebasic molt. J Exp Zool. 2011;315A:512–9.
Li D, Zhang X, Li Y, Hao C, Zhang J, Wu Y. Stress responses of testosterone and corticosterone-binding globulin in a multi-brooded species, Eurasian Tree Sparrows (Passer montanus): does CBG function as a mediator? Horm Behav. 2012;61:582–9.
Li M, Sun Y, Wu J, Zhang X, Li J, Yao Y, Liu X, Li D, Wu Y. Variation in corticosterone response and corticosteroid binding-globulin during different breeding sub-stages in Eurasian tree sparrow (Passer montanus). J Exp Zool. 2016;325A:75–83.
Lynn SE, Stamplis TB, Barrington WT, Weida N, Hudak CA. Food, stress, and reproduction: short-term fasting alters endocrine physiology and reproductive behavior in the zebra finch. Horm Behav. 2010;58:214–22.
Mason G, Burn CC, Dallaire JA, Kroshko J, Kinkaid HM, Jeschke JM. Plastic animals in cages: behavioural flexibility and responses to captivity. Anim Behav. 2013;85:1113–26.
Mason GJ. Species differences in responses to captivity: stress, welfare and the comparative method. Trends Ecol Evol. 2010;25:713–21.
Morgan KN, Tromborg CT. Sources of stress in captivity. Appl Anim Behav Sci. 2007;102:262–302.
Mori JG, George JC. Seasonal changes in serum levels of certain metabolites, uric acid and calcium in the migratory Canada goose (Branta canadensis interior). Comp Biochem Physiol B. 1978;59:263–9.
Muldoon MF, Kritchevsky SB, Evans RW, Kagan VE. Serum total antioxidant activity in relative hypo-and hypercholesterolemia. Free Radic Res. 1996;25:239–45.
Orgebin-Crist MC, Tichenor PL. Effect of testosterone on sperm maturation in vitro. Nature. 1973;245:328–9.
Partonen T, Haukka J, Virtamo J, Taylor PR, Lonnqvist J. Association of low serum total cholesterol with major depression and suicide. Br J Psychiatry. 1999;175:259–62.
Rich EL, Romero LM. Exposure to chronic stress downregulates corticosterone responses to acute stressors. Am J Physiol Reg I. 2005;288:R1628–36.
Romero LM. Seasonal changes in plasma glucocorticoid concentrations in free-living vertebrates. Gen Comp Endocrinol. 2002;128:1–24.
Romero LM, Wingfield JC. Alterations in hypothalamic–pituitary–adrenal function associated with captivity in Gambel’s white-crowned sparrows (Zonotrichia leucophrys gambelii). Comp Biochem Physiol B. 1999;122:13–20.
Sapolsky RM, Romero LM, Munck AU. How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocr Rev. 2000;21:55–89.
Sautin YY, Johnson RJ. Uric acid: the oxidant-antioxidant paradox. Nucleosides Nucleotides Nucl. 2008;27:608–19.
Schroepfer GJ. Oxysterols: modulators of cholesterol metabolism and other processes. Physiol Rev. 2000;80:361–554.
Shmueli M, Izhaki I, Zinder O, Arad Z. The physiological state of captive and migrating Great White Pelicans (Pelecanus onocrotalus) revealed by their blood chemistry. Comp Biochem Physiol A. 2000;125:25–32.
Stinefelt B, Leonard SS, Blemings KP, Shi X, Klandorf H. Free radical scavenging, DNA protection, and inhibition of lipid peroxidation mediated by uric acid. Ann Clin Lab Sci. 2005;35:37–45.
Strazzullo P, Puig JG. Uric acid and oxidative stress: relative impact on cardiovascular risk. Nutr Metab Cardiovasc. 2007;17:409–14.
Summers-Smith D. Eurasian tree sparrow (Passer montanus). In: del Hoyo J, Elliott A, Sargatal J, Christie DA, de Juana E, editors. Handbook of the birds of the world. Barcelona: Lynx Edicions; 2014.
Tift MS, Houser DS, Crocker DE. High-density lipoprotein remains elevated despite reductions in total cholesterol in fasting adult male elephant seals (Mirounga angustirostris). Comp Biochem Physiol B. 2011;159:214–9.
Tsahar E, Arad Z, Izhaki I, Guglielmo CG. The relationship between uric acid and its oxidative product allantoin: a potential indicator for the evaluation of oxidative stress in birds. J Comp Physiol B. 2006;176:653–61.
Vegiopoulos A, Herzig S. Glucocorticoids, metabolism and metabolic diseases. Mol Cell Endocrinol. 2007;275:43–61.
Wingfield JC, Kitaysky AS. Endocrine responses to unpredictable environmental events: stress or anti-stress hormones? Integr Comp Biol. 2002;42:600–9.
Wingfield JC, Maney DL, Breuner CW, Jacobs JD, Lynn S, Ramenofsky M, Richardson RD. Ecological bases of hormone-behavior interactions: the “emergency life history stage”. Am Zool. 1998;38:191–206.
Wingfield JC, O’Reilly KM, Astheimer LB. Modulation of the adrenocortical responses to acute stress in arctic birds: a possible ecological basis. Am Zool. 1995;35:285–94.
Wingfield JC, Vleck CM, Moore MC. Seasonal changes of the adrenocortical response to stress in birds of the Sonoran Desert. J Exp Zool. 1992;264:419–28.
Yamada F, Inoue S, Saitoh T, Tanaka K, Satoh S, Takamura Y. Glucoregulatory hormones in the immobilization stress-induced increase of plasma glucose in fasted and fed rats. Endocrinology. 1993;132:2199–205.
Zhao Y, Gong L, Zhao B, Gao X, Wu Y, Li D. Life-history dependent relationships between plasma alkaline phosphatase activity and body condition in male Eurasian Tree Sparrows. Avian Res. 2017a;8:18.
Zhao Y, Li M, Sun Y, Wu W, Kou G, Guo L, Xing D, Wu Y, Li D, Zhao B. Life-history dependent relationships between body condition and immunity, between immunity indices in male Eurasian tree sparrows. Comp Biochem Physiol A. 2017b;210:7–13.
We are grateful to Yao Yao, Simeng Yu, Dan Liu, Li Zhang, Chao Du for expert assistance in the field, and two anonymous reviewers for their helpful comments on the original manuscript.
Ethics approval and consent to participate
All procedures were approved by the Institutional Animal Care and Use Committee of Hebei Normal University and carried out under the auspices of scientific collecting permits issued by the Hebei Provincial Forestry Bureau’s Department of Wildlife Conservation.
Consent for publication
The authors declare that they have no competing interests.