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Egg-spot matching in common cuckoo parasitism of the oriental reed warbler: effects of host nest availability and egg rejection

  • Donglai Li†1,
  • Yanan Ruan†1,
  • Ying Wang1,
  • Alan K. Chang1,
  • Dongmei Wan1Email author and
  • Zhengwang Zhang2
†Contributed equally
Avian Research20167:21

https://doi.org/10.1186/s40657-016-0057-y

©  The Author(s) 2016

Received: 5 August 2016

Accepted: 9 November 2016

Published: 21 November 2016

Abstract

Background

The success of cuckoo parasitism is thought to depend largely on the extent of egg matching between cuckoo and host eggs, since poor-matching cuckoo egg would lead to more frequent egg rejection by the host. In this study, we investigated how egg-spot matching between the Common Cuckoo (Cuculus canorus) and its host, the Oriental Reed Warbler (Acrocephalus orientalis) is affected by the local parasitism rate, nest availability in breeding synchronization and egg rejection.

Methods

We used the paired design of parasitized and their nearest non-parasitized nests where breeding occurred simultaneously to compare egg-spot matching. The image analysis was used to compare four eggshell pattern variables, namely spot size, density, coverage on the different areas of egg surface, and the distribution on the whole egg surface. Egg recognition experiments were conducted to test the effect of egg spots on egg rejection by the host.

Results

Our results show that much better matching in almost all spot parameters tested on the side of the egg and the spot distribution on the whole egg occurred in parasitized nests than in non-parasitized nests. Matching of spot density between cuckoo and host eggs in parasitized nests increased with the synchronization between temporal availability of nests and the egg-laying period of female cuckoos. Egg recognition experiments in which the warbler eggs were deliberately painted with extra spots led to a significantly higher egg rejection rate (78.3%) than of unpainted eggs.

Conclusion

Our data suggest that both the high temporal encounter rate between cuckoo and warbler nests as well as the high egg rejection ability of the host are important factors for egg-spot matching of the cuckoos.

Keywords

Brood parasiteCuckooEgg mimicryEgg matchingSpot patternEgg rejectionParasitism rateOriental Reed Warbler

Background

Mutual interactions between parasitic cuckoos and their hosts represent an outstanding textbook example of co-evolution (Rothstein 1990; Feeney et al. 2014). Such interactions have led to the evolution of many amazing biological phenomena (Davies 2011; Spottiswoode and Stevens 2012; Soler 2014). Among these, the remarkable resemblance in egg appearance (color and spot patterns) between cuckoos and their hosts, termed “mimicry”, has long attracted the attention of behavioral ecologists (Chance 1940; Brooke and Davies 1991; Davies 2000; Igic et al. 2011). Although this phenomenon has been extensively studied, it has largely been explained as an evolutionary response to the strong egg rejection carried out by the host during their long co-evolutionary interaction (Brooke and Davies 1988; Soler et al. 2003; Stoddard and Stevens 2011; Avilés et al. 2012). However, the effect that active host-nest selection has on egg mimicry used by the cuckoo partner has not been conclusively demonstrated (see Avilés and Møller 2004). The Common Cuckoo (Cuculus canorus, hereafter “the cuckoo”), one of the most well studied obligate brood parasites, has many different egg phenotypes, called “gentes” or “races” that are distinguishable among each other and show host-specific lineages (Moksnes and Røskaft 1995; Marchetti et al. 1998; Gibbs et al. 2000; Fossøy et al. 2011, 2016). The existence of these gentes is probably maintained by the tendency of the cuckoo to choose its host actively, as a result of host imprinting, a phenomenon that has previously been reported by various investigators (Wyllie 1981; Honza et al. 2001, 2002; Avilés and Møller 2004; Nakamura et al. 2005). However, how individual cuckoos specifically choose their hosts within a single species of birds, in particular following the matching between their own eggs and those of their hosts, has been a subject of great debate (Antonov et al. 2012; Yang et al. 2015b).

There are two hypotheses regarding whether the cuckoo can actively choose the host nests which bear eggs with similar appearance to its own eggs during parasitism. In recent years, several empirical studies have been conducted to investigate these two interesting hypotheses, but the results from these studies are rather ambiguous. The egg-matching hypothesis (EMH) suggests that the cuckoo lays eggs matching the appearance of the eggs of its host because the high rejection rate of poorly matched eggs would be costly to its breeding process (Avilés et al. 2006; Cherry et al. 2007; Feeney et al. 2012; Honza et al. 2014). Obviously, this egg-laying strategy would increase the possibility of egg acceptance by the hosts and improve the evolutionary progress of egg mimicry. However, it is still unclear if a female cuckoo can recognize its own egg phenotype and has the ability to choose the right nest out of the many nests it encounters and, if so, the problem arises whether there are always enough suitable nests (in the egg laying stage before incubation) available to be used within its breeding territory (Liang et al. 2016). The random egg-laying hypothesis (REH) states that brood parasites lay eggs randomly in host nests (Orians et al. 1989; Antonov et al. 2012), because its secretive and rapid egg-laying behavior limits the visual discrimination of their own egg appearance and that of the host eggs (Payne 1977; Yang et al. 2015b). Besides, it is very common for a cuckoo to lay eggs in the nests of the hosts that have different egg appearances (Moksnes and Røskaft 1995; Grim 2002). Recently, Yang et al. (2015b) have used a conceptual model to argue that the egg-matching egg-laying behavior is maladaptive in the case of the Common Cuckoo and its parrotbill hosts, both of which have evolved to lay distinctive polymorphic eggs. This phenomenon has further been confirmed in the Tailorbirds (Orthotomus sutorius), which are also randomly parasitized by Plaintive Cuckoos (Cacomantis merulinus), irrespective of the appearance of their eggs (Yang et al. 2015a).

Three empirical studies from Europe have shown that cuckoo eggs are more similar to the host eggs in naturally parasitized nests than in non-parasitized or randomly chosen nests, thereby providing support for the EMH (Avilés et al. 2006; Cherry et al. 2007; Honza et al. 2014). These studies have focused more on the background color of the eggs and less attention has been given to the spot pigment and pattern of the eggs. However, recent studies (Antonov et al. 2012; Šulc et al. 2016; Yang et al. 2016), which focused on the cuckoo hosts, the Marsh Warbler (Acrocephalus palustris), the Great Reed Warbler (A. arundinaceus) and the Oriental Reed Warbler (A. orientalis), have found no support for the EMH, despite the inclusion of egg-spot traits. Several studies have suggested that egg color is a more important recognition cue than spot patterns (Moskát et al. 2008; Spottiswoode and Stevens 2010). However, relative to egg color, the spottiness of the egg is thought to have a greater variation and is more distinctive and complex, thus having more recognizable signatures that are more likely to be involved in egg recognition carried out by the hosts (Øien et al. 1995; Kilner 2006; Underwood and Sealy 2006; Stoddard and Stevens 2010; Spottiswoode and Stevens 2012; Medina et al. 2016).

From these studies, it can be concluded that the two hypotheses might not be mutually exclusive. Recently, one model study has shown that both EMH and REH are valid for cuckoo parasitism in the presence of egg polymorphism (Liang et al. 2016). The choice of which egg-laying strategy would be adopted can be determined by many ecological and evolutionary changes, both in the cuckoo and its host, but most of these have largely been neglected in previous studies. Two critical factors known to determine which strategy is optimal are the rate of encounter with the host nests and the level of egg rejection achieved by the host (Liang et al. 2016). The parasitism rate can partly reflect the rate of encounter with host nests (population density of cuckoo and/or the availability of host nests) (Stokke et al. 2007), but this parameter is rarely used to investigate its effect on the egg-laying strategy of cuckoos (but see Soler et al. 2015). In addition, the same cuckoo, either as an individual bird or as a population, may also shift the choice of egg-laying strategy following the temporal availability of host nests vulnerable to parasitism.

The Oriental Reed Warbler (Acrocephaus orientalis) is frequently parasitized by the Common Cuckoo in eastern China (Liang et al. 2014; Yang et al. 2014), where both species lay dark, brown-spotted eggs (Li et al. 2016). We used egg spots as the main feature to investigate the phenomenon of egg matching between cuckoo and warbler and its effect on egg rejection conducted by the warbler. We speculated that cuckoos may choose the EMH egg-laying strategy if the egg spots can act as a significant signature that decides whether the egg is accepted or rejected by the warbler, or whether there is a high availability of suitable host nests at the time the cuckoo lays its egg. Furthermore, we also hypothesized that egg-spot matching between cuckoo and warblers would increase with more nest synchronization at the cuckoo egg-laying stage, if the cuckoos temporally fine tune their egg-laying strategy to EMH or REH within one breeding season. We also discuss other factors that may affect the choice of alternative strategies.

Methods

Study site and field measurements

Field work was carried out during the breeding season (late May to July) in 2013-2015, at the Liaohe Delta Nature Reserve (41.033929°N; 121.725244°E), Liaoning Province, northeastern China. This region has a semi-arid continental monsoon climate with rainfall usually occurring in late July to September. The delta is one of the most important estuarine wetlands in the coastal region of China, with the largest area of Phragmites reed fields (800 km2) in northeastern Asia. These Phragmites wetlands are extensively used for culturing Chinese Mitten Crabs (Eriocheir sinensis) and for the production of reed biomass used in the paper industry. As well, energy projects are being constructed in these wetlands because of the oil fields they contain. The associated oil field infrastructures have led to the installation of electrical wires, which provide perch sites for the cuckoos. Significant growth of these wetlands is found along the edge of the reed fields along ditches, which have become a suitable nesting place for the Oriental Reed Warblers (hereafter “the warbler”). The warbler is the dominant host for the cuckoo in this area. Both the cuckoo and warbler are migrant birds. They come to breed in these wetlands in large numbers during late May to early June. The breeding season lasts till early August.

We systematically searched for nests of warblers at three main reed fields (<5 km between each site), where the warblers breed in high densities. After a nest was found, its location was recorded with a Garmin GPS 60s. Almost all the nests were found during the nest-building or egg-laying stage and were checked daily until clutch completion. During each check, every newly laid egg was numbered using a felt tip pen to allow the identification of missing eggs. To investigate whether the cuckoos would actively choose between nests that are equally accessible, we used the paired design of parasitized and their nearest non-parasitized nests where breeding occurred simultaneously (i.e., the interval of egg-laying dates is ≤5 days) with the distance between the two nests less than 150 m (Antonov et al. 2012; Yang et al. 2016). These criteria were chosen because the warbler has a typical clutch size of five eggs and is most often parasitized during the 5-day egg-laying stage. The warbler lays one egg per day at dawn and previous video recordings have confirmed that female cuckoos tend to remove one warbler egg from the nest that they parasitize (n = 7; Li et al. unpublished data), which is similar to the laying behavior of the Common Cuckoo in Europe (Davies and Brooke 1988). To enable the correct assignment of the parasitism status, only nests found during the nest-building or early egg-laying stage (1‒2 eggs) were included (Antonov et al. 2012). These nests were monitored daily until clutch completion. One cuckoo egg was found to be rejected four days after it was laid. Fortunately, this nest was photographed before the egg was ejected and used in the analysis. The rejection rate (including nest desertion) of cuckoo eggs was lower than 9% (n = 35) according to our previous unpublished data, a much lower rate than the 26.9% reported for the Great Reed Warbler in Europe (Kleven et al. 2004). No marked warbler eggs were missing except for two non-parasitized nests in which the missing marked eggs were exchanged with cuckoo eggs during the monitoring period and were therefore excluded from the analysis. Only nests parasitized by one cuckoo egg were used in our study. Subsequently, all the eggs of two clutches within a pair were photographed (Cannon D70 camera) in a standard way on a Kodak grey plate. In order to show the spot distribution on the entire egg, we photographed the spot distribution on the blunt and pointy ends as well as on the side of the egg (see Fig. 1). During the taking of photographs, we used a blind method to ensure a random side of the eggs was shown (Yang and Liang 2016).
Figure 1
Fig. 1

Egg-spot pattern as viewed from different angles of the egg. The black and white images were obtained with the ImageJ software. The region on the egg enclosed by yellow circle represents about half the visible area used in the analysis

Egg-spot measurements

All the egg photographs were processed using the ImageJ software (version 1.45 s, http://imagej.nih.gov/ij). These images were first balanced according to a white standard on the color scale and then the areas containing the egg images were cropped and scaled. We used Bernsen’s threshold method to convert the images into a binary (black/white) format, with black pixels corresponding to the egg spots and white pixels to the background egg color (Landini 2011). Circular frames were selected to represent the blunt and pointy ends of the egg whereas an ellipse frame was selected to represent the side of the egg. Only about half of the visible area photographed in the central part of the egg was selected for subsequent spot pattern analyses, because the margin of the image could be biased due to the curvature of the egg and unequal shading (Šulc et al. 2016). According to the protocol in Antonov et al. (2012), we extracted four variables for the spot patterns: (1) spot size (mm2)—the average value for all spots within the selected frame; (2) spot density (number of spots/mm2)—number of spots per unit area; (3) spot coverage (%)—percentage area of the egg covered by spots; (4) spot distribution—proportion of spot coverage at the blunt end. Finally, the absolute differences among the four spotting-pattern variables were calculated for each cuckoo egg in relation to the host eggs of the parasitized and non-parasitized nests and used as indices of contrast. We used two methods to compare the spot contrast between parasitized and non-parasitized nests. First, the method used by Antonov et al. (2012) was adopted, in which one random host egg was selected from each nest in the blind method and the difference between this egg and the cuckoo egg was assessed. To avoid a potential deficiency in the method used by Antonov et al. (2012), which is caused by intra-clutch variation of the host eggs, a similar assessment to that reported by Yang et al. (2016) was also performed, but the average value of the whole clutch was used instead of the average value of a single egg.

Egg recognition experiments

Several studies have pointed out the significance of the effect of spot pattern on egg recognition by the hosts (Hauber et al. 2006; Moskát et al. 2008, 2014). Therefore, we also conducted egg recognition experiments to confirm the importance of spot matching in this cuckoo-warbler system. We used 43 non-parasitized host nests in this study and randomly divided them into two groups in 2014‒2015. In the experimental group, one host egg from the experimental nest was randomly chosen after the whole clutch had been laid. Ten extra dark brown spots, each about 3‒4 mm in diameter, were then marked on the chosen egg using a brown fiber pen, which produced a similar natural dark spot color to that of the existing spots (Additional file 1: Fig. S1). In the control group, one egg was also randomly selected and manipulated in the same manner, but the egg was only marked with a number signature using a black water-proof pen. The nests were checked either daily or every second day. When the marked egg in the nest was missing within six days, it was assumed to be the result of rejection, whereas if the marked egg was still incubated in the nest at this time, it was considered as accepted by the host (Moksnes et al. 1991). No nest desertion was discovered, and nests that were predated during the six days were omitted.

Data manipulation and statistical analyses

The parasitism rate was presented as the percentage of nests found to contain cuckoo eggs or chicks. The first egg-laying date was recorded during the daily nest-checks or back-calculated from the number of eggs laid. The distance between nests as well as the distance from the nests to the nearest cuckoo perches (i.e., electric wires) were measured by the GoodyGIS (version 3.24) using the GPS location data of the nests. As suggested by Honza et al. (2014), it is really difficult to determine the home range at which a particular female cuckoo would lay egg; previous studies have revealed equivocal evidence for an exclusive territorial defense by female cuckoos. Thus we viewed all nests with similar egg-laying timing as nest availability to assess the potential encounter rate at which the cuckoo encountered its host nest when the cuckoo laid its egg. The potential host nests that were readily accessible to cuckoo parasitism were determined by tabulating the number of nests with the egg-laying phase commencing within five days before the cuckoo laid its egg, because the typical clutch size of the warbler is five eggs and they are most frequently parasitized by cuckoos in this period.

Chi square tests were used to compare the annual variation in parasitism rates and egg rejection rates by the host among groups. The differences in the first egg-laying date, the distance to perch site and the contrast in the spot pattern between parasitized and non-parasitized nests were compared using paired sample t-tests when normalization of the data was obtained. Otherwise, Wilcoxon signed rank tests were employed. We used independent two sample t-tests to compare the number of nests with synchronized egg-laying between the parasitized nests used for spot assessment and parasitized nests that were not assessed. Linear regression was used to explore the relationship between the spot contrast index (between cuckoo and warbler eggs) in the parasitized nests and nest availability with similar egg-laying dates. All statistical analyses were performed with Sigmaplot version 11.0. Data were presented as mean ± SE and statistical significances were considered at the p < 0.05 level.

Results

Parasitism rates and sample size for measurement of egg spots

The local cuckoo parasitism rate on the warbler was 16.6% (n = 373, range 14.7‒18.6%), without evidence of an annual fluctuation (Chi square test: χ 2 = 0.815, df = 2, p = 0.665; Additional file 2: Table S1). Only 1.3% (n = 373) of the nests were parasitized by two cuckoo eggs, with an annual variation of one to two nests. According to a strict paired design criterion, 39 pairs of parasitized and non-parasitized nests were successfully obtained, with an average distance, between two nests within each pair, of 53.2 ± 5.1 m (8.8‒147.4 m). The average nest availability for the parasitized nests used for spot assessment was 28.46 ± 2.48 (SE), range 5‒57 (n = 39), which did not differ significantly from the 23 parasitized nests that were not assessed (22.42 ± 2.57; t = 1.61, df = 61, p = 0.112). No significance differences were found between parasitized and non-parasitized nests, either in the starting egg-laying dates of the host (first egg-laying date for the hosts in parasitized vs. non-parasitized nests: date 1 is June 1; 11.62 ± 6.85 vs. 11.28 ± 6.30; t = 0.763, df = 38, p = 0.450) or in the distance to the nearest perch (parasitized vs. non-parasitized nests: 33.10 ± 52.43 vs. 40.53 ± 61.51; Z = 1.180, df = 38, p = 0.241).

Matching egg-spot patterns between cuckoos and their hosts

Measurements taken for egg-spot parameters on the side of the egg revealed significantly smaller contrasts between cuckoo eggs and host eggs in parasitized nests than in non-parasitized nests, with respect to spot size and spot density, both from the one-egg (Fig. 2) and the whole clutch assessments (Additional file 3: Fig. S2). In spot coverage, the contrast between cuckoo and host eggs was also significant on the basis of one-egg assessment, but not statistically significant in the case of whole clutch assessment, although the contrast was still obvious. No significant contrasts between cuckoo and host eggs in terms of egg spots on the blunt and sharp ends of the eggs were found, either on the basis of one-egg or on whole clutch assessments (Table 1). In the case of spot distribution, both types of assessments revealed significantly smaller contrasts in the parasitized nests (Fig. 2; Additional file 3: Fig. S2).
Figure 2
Fig. 2

Comparison of egg-spot matching between cuckoo and host eggs in parasitized and nearest non-parasitized nests. The plots show the medians, quartiles, 5th and 95th percentiles of the spot contrasts. Only results for the one-egg assessment are presented. The data from the whole-clutch assessment yielded similar conclusions and are therefore shown as supplementary material

Table 1

Comparison of egg-spot (spot size, spot density, spot coverage and sport distribution) matching between cuckoo and warbler eggs in naturally parasitized nests and their nearest non-parasitized nests

Assessment method

Egg section

Variable

Parasitized

Non-parasitized

Statistics

One-egg assessment

Sharp

Spot size (mm2)

0.21 ± 0.18

0.17 ± 0.19

t = 1.308, df = 38, p = 0.199

  

Spot density (No. of spots/mm2)

0.37 ± 0.47

0.48 ± 0.66

Z = 0.865, df = 38, p = 0.391

  

Spot coverage (%)

17.37 ± 11.38

17.84 ± 14.98

t = 0.163, df = 38, p = 0.871

 

Blunt

Spot size (mm2)

0.10 ± 0.14

0.24 ± 0.49

Z = 1.283, df = 38, p = 0.202

  

Spot density (No. of spots/mm2)

0.37 ± 0.43

0.42 ± 0.40

Z = 1.284, df = 38, p = 0.202

  

Spot coverage (%)

8.46 ± 8.70

12.61 ± 20.43

Z = 1.102, df = 38, p = 0.273

The whole clutch assessment

Sharp

Spot size (mm2)

0.21 ± 0.18

0.17 ± 0.19

t = 1.321, df = 38, p = 0.194

  

Spot density (No. of spots/mm2)

0.36 ± 0.39

0.43 ± 0.62

Z = 0.670, df = 38, p = 0.507

  

Spot coverage (%)

17.26 ± 11.45

16.99 ± 14.20

t = 0.107, df = 38, p = 0.916

 

Blunt

Spot size (mm2)

0.07 ± 0.08

0.20 ± 0.39

Z = 1.493, df = 38, p = 0.137

  

Spot density (No. of spots/mm2)

0.45 ± 0.66

0.40 ± 0.39

Z = 0.670, df = 38, p = 0.507

  

Spot cover (%)

8.86 ± 12.77

12.20 ± 19.36

Z = 1.109, df = 38, p = 0.273

Only assessments on the sharp and blunt end of the egg are shown. Data are means ± SEs (n = 39)

Relationship between egg-spot matching and temporal availability of host nests

Spot density contrasts on the side (F 1,37 = 7.44, p = 0.010), blunt (F 1,37 = 9.21, p = 0.004) and sharp (F 1,37 = 0.353, p = 0.028) ends of the egg between cuckoo and warbler parasitized nests were negatively correlated with nest availability in the one-egg assessment (Fig. 3; Table 2). Similarly, a negative correlation was also observed for the other spot contrasts on the side of the egg in the one-egg assessment, but the correlation was not significant (Fig. 3). Similar results were also obtained for the whole-clutch assessment, with spot density contrasts between cuckoo eggs and warbler eggs in the parasitized nests negatively correlated with nest availability on the side (F 1,37 = 7.53, p = 0.009) and blunt end of the egg (F 1,37 = 4.26, p = 0.046), but not on the sharp end of the egg (F 1,37 = 2.87, p = 0.099). Additional information can be found in Additional file 4: Fig. S3 and Table 2.
Figure 3
Fig. 3

Correlation between egg-spot matching (between cuckoo and warbler eggs) in parasitized nests and availability of host nests, synchronized with cuckoo egg-laying. The data represent spot contrasts. The solid and dashed lines show linear regressions and their 95% confidence intervals, respectively. Only results for the one-egg assessment are presented. Data from the whole clutch assessment yielded the same conclusion and are shown as supplementary materials

Table 2

Correlation between egg-spot matching (between cuckoo and warbler eggs) in parasitized nests and availability of host nests synchronized with cuckoo egg-laying

Assessment method

Egg section

Variables

Linear regression equation

R

F

p

One-egg assessment

Side

Spot size (mm2)

Spot size = 0.198 − (0.00189 × nest availability)

0.137

0.704

0.407

  

Spot density (No. of spots/mm2)

Spot density = 0.418 − (0.00575 × nest availability)

0.409

7.436

0.01

  

Spot coverage (%)

Spot coverage = 10.412 − (0.0918 × nest availability)

0.229

2.048

0.161

 

Sharp

Spot size (mm2)

Spot size = 0.168 + (0.00150 × nest availability)

0.128

0.618

0.437

  

Spot density (No. of spots/mm2)

Spot density = 0.679 − (0.0108 × nest availability)

0.353

5.255

0.028

  

Spot coverage (%)

Spot coverage = 18.364 − (0.0349 × nest availability)

0.0475

0.0837

0.774

 

Blunt

Spot size (mm2)

Spot size = 0.0955 + (0.000157 × nest availability)

0.0171

0.0109

0.918

  

Spot density (No. of spots/mm2)

Spot density = 0.721 − (0.0124 × nest availability)

0.446

9.207

0.004

  

Spot coverage (%)

Spot coverage = 12.079 − (0.127 × nest availability)

0.227

2.004

0.165

  

Spot distribution

Spot distribution = 11.586 − (0.124 × nest availability)

0.219

1.867

0.18

The whole clutch assessment

Side

Spot size (mm2)

Spot size = 0.191 − (0.00141 × nest availability)

0.0912

0.31

0.581

  

Spot density (No. of spots/mm2)

Spot density = 0.522 − (0.00642 × nest availability)

0.411

7.534

0.009

  

Spot coverage (%)

Spot coverage = 12.154 − (0.127 × nest availability)

0.269

2.886

0.098

 

Sharp

Spot size (mm2)

Spot size = 0.0700 + (0.0000734 × nest availability)

0.015

0.0083

0.928

  

Spot density (No. of spots/mm2)

Spot density = 0.775 − (0.0114 × nest availability)

0.268

2.87

0.099

  

Spot coverage (%)

Spot coverage = 11.563 − (0.0948 × nest availability)

0.115

0.496

0.486

 

Blunt

Spot size (mm2)

Spot size = 0.177 + (0.00106 × nest availability)

0.0892

0.297

0.589

  

Spot density (No. of spots/mm2)

Spot density = 0.588 − (0.00799 × nest availability)

0.321

4.261

0.046

  

Spot coverage (%)

Spot coverage = 19.542 − (0.0802 × nest availability)

0.108

0.441

0.511

  

Spot distribution

Spot distribution = 0.412 + (0.0131 × nest availability)

0.203

1.593

0.215

A total of 38 DFs were used in the analysis

Effect of egg spots on the rejection rate by hosts

The egg-rejection rate in the nests that contained the warbler egg painted with ten extra brown spots was 78.3% (n = 23), which was significantly higher than that in the control group (0%, n = 20; χ 2 = 23.8, df = 1, p < 0.0001). All the spotted eggs were ejected with no loss and damage to the unmarked eggs.

Discussion

Our study has revealed partial, but clear evidence supporting the presence of better egg-matching in spot patterns (spot size, spot density, spot coverage and spot distribution) between cuckoo and warbler eggs in parasitized nests than in non-parasitized nests, despite some deficiencies in the methodology and the hypothesis itself, such as the fact that cuckoos generally peck one or more host eggs during parasitism, while they have no opportunity to assess the whole-clutch (see discussion in Yang et al. 2016). The study has also provided large amounts of important experimental data in attempting to clarify the mixed and contrasting hypotheses on cuckoo egg-matching laying behavior. Previous studies conducted by Antonov et al. (2012) and Yang et al. (2016) on cuckoos have found no support for the EMH based on egg-spot matching. Several factors may have accounted for the different outcomes in these studies. First, although we all used similar paired designs, Antonov et al. (2012) obtained their results based only on a comparison with the randomly selected host egg rather than the whole clutch. Second, Yang et al. (2016) compared the overall egg-spot pattern using the Nature-Pattern-Match (NPM) model developed by Stoddard et al. (2014) rather than specific aspects of the egg-spot pattern as in our case. Although this NPM model has recently been recommended and the granularity filtering approach pattern processing used (e.g. Spottiswoode and Stevens 2010; Stoddard and Stevens 2010; Spottiswoode and Stevens 2012; Stoddard et al. 2014; Caves et al. 2015; Medina et al. 2016), spot measurement is still a commonly used approach (Gosler et al. 2000; Antonov et al. 2012). Third, we used a larger sample size (n = 39) than either of these two studies (n = 20: Antonov et al. 2012 and n = 30: Yang et al. 2016) and thus our result should be statistically more robust. Finally, as suggested in a study by Liang et al. (2016), one of the most important factors determining the choice of egg-laying strategy is the rate at which a cuckoo encounters a host nest. The local parasitism rate, nest site distribution and host behavior could all be related to the accessibility of the host nest and cuckoo-host-nest encounter rate. At the site where we carried out the experiment, the parasitism rate was found to be 16.9%, compared to 65.5% in the case of Yang et al. (2016). A higher parasitism rate means a shortage of available nests to be parasitized, which may translate into a smaller opportunity for the cuckoo to select the nest during the process of parasitism. A lack of selection opportunity is also evident in the study by Antonov et al. (2012), since the Marsh Warbler nests were sparsely distributed and most were also inconspicuous to the cuckoos, thus compromising their nest selection based on egg-spot matching. By reviewing all previous empirical data (Additional file 5: Table S2), we found that these factors could well explain almost all inferences drawn by earlier studies. All published data supporting the EMH were gathered from noisy hosts, which have a relatively low parasitism rate, i.e., between 11.3 and 31.1% (Avilés et al. 2006; Honza et al. 2014), except for the study by conducted by Cherry et al. (2007), in which a parasitism rate of 64% was recorded, but these authors did not use the paired design to test the egg-matching between natural parasitized nests and non-parasitized nests. All other empirical data in support of the REH can either be explained by relatively high parasitism rates (Yang et al. 2016) or is due to the inconspicuous behavior of the hosts, which limits the opportunity for the cuckoo to select the best nest (Antonov et al. 2012; Yang et al. 2015a, b).

Except for the partially clear spot matching between cuckoo egg and the egg of its chosen host mentioned earlier, we also found that the spot density contrast between cuckoo and host eggs in the parasitized nests displayed significant negative correlation with the number of available host nests. This implied that the egg-matching egg-laying strategy of cuckoos could also vary and may change with fluctuations in the temporal availability of host nests. The effect of breeding synchronization among the host on the risk of cuckoo parasitism and nest-searching strategy has been reported (e.g. Jelínek et al. 2014; Soler et al. 2015), but our result provided evidence for the effect of breeding synchronization among the hosts on the egg-matching laying strategy adopted by cuckoos. This result further implied that the laying strategy could be fine-tuned in a relatively short period within one breeding season.

Another important factor that could also affect the egg-matching egg-laying strategy of the cuckoo is the egg rejection ability of host as suggested by Liang et al. (2016). The Oriental Reed Warbler, which is similar to its sister, the Great Reed Warbler of Europe, displays relatively high egg discrimination towards cuckoo eggs (Lotem et al. 1995; Moskát and Honza 2002; Li et al. 2016). However, the cognitive signature (either from the background color or/and egg spot) involved in the recognition process is not clearly understood. Although egg color has been shown to play an essential role (Moskát et al. 2008; Spottiswoode and Stevens 2010; Yang et al. 2010), several previous studies have reported that egg spots are more important than other egg phenotypes (e.g., egg color) for self recognition and for distinguishing non-self eggs (López-de-Hierro and Moreno-Rueda 2010; Stoddard and Stevens 2010; Stoddard et al. 2014). In our case, we found that egg rejection by warblers was as high as 78.3% when the warbler eggs were artificially painted with ten extra spots. Although the spots deliberately painted on the egg could not represent the natural spot pattern of the egg, this manipulation is commonly used to test the effect of egg patterns on egg rejection by warbler hosts (Hauber et al. 2006; Moskát et al. 2008, 2014). Therefore, our study clearly confirms that egg-spot patterns play an important role in egg rejection by the Oriental Reed Warbler, consequently leading to high selection pressure for the cuckoo to evolve a better matching egg-laying strategy.

Several aspects of the egg-spot pattern were considered in our egg spot-matching experiments, i.e., spot size, spot density, spot coverage and spot distribution. Except for spot coverage, which showed somewhat lower matching, the rest showed significant matching, either in the single egg or in the clutch analysis (Fig. 2; Additional file 3: Fig. S2). Furthermore, matching between cuckoo and warbler eggs appeared to occur on the side of the egg rather than on either end of the egg. Since the position of the egg inside the nest is such that the most exposed surface of the egg is the side (Polačiková et al. 2013), it becomes clear that matching on the side of the egg is more important to ensure the successful egg-laying strategy of cuckoo. However, other investigations have found that spot patterns on the blunt end of the egg constitute an important egg-pattern signature (Polačiková et al. 2007, 2010; Polačiková and Grim 2010). In the case of cuckoo eggs, the overall spot pattern on the blunt end exhibited lower matching with patterns on the blunt end of warbler eggs (Additional file 6: Supplementary Document 1). However, the eggs of both species have a relatively high spot density on the blunt end, thus making it difficult for the warbler to distinguish any differences in spot sizes on the non-self egg (Stoddard et al. 2014).

In conclusion, we demonstrated that the Common Cuckoo egg-laying matching in egg-spot pattern varied depending on the temporal availability of host nests. We propose that the host selection strategy of the Common Cuckoo probably involves both egg-phenotype matching and non-matching, not only in a parasitism system in which egg phenotypes exhibit polymorphism (Liang et al. 2016), but also in a parasitism system that exhibits continuous inter-clutch egg phenotypes. The choice which strategy to use may largely depend on both the condition of the environment (e.g., nest availability and conspicuousness) and egg rejection by the host.

Notes

Declarations

Authors’ contributions

DL, YR, DW, ZZ designed the experiments. DL, YW participated in the field work and data analysis. DL and YR wrote the manuscript and AKC helped to improve the structure and language of the manuscript. All authors have read and approved the final manuscript.

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 31301888 and 31672316 to DL), the General Scientific Research Project of the Education Department of Liaoning Province (L2015196 to DL) and the Open Fund of the Ministry of Education Key Laboratory for Biodiversity Sciences and Ecological Engineering, Beijing Normal University (K1401 to DL). We are grateful to two anonymous referees for constructive comments that significantly improved the manuscript. We would like to thank Yuxiang Li and Changbo Fu from the Liaohe Delta National Nature Reserve Management Bureau for their permission to undertake this study. We are also grateful to Elyse Iemola, Lei Zhang, Hongwei Wei, Xinghai Sun, Qingbin Wang and Long Guo for their assistance in the fieldwork.

Competing interests

The authors declare that they have no competing interests.

Ethical standards

The experiments complied with the current laws of China. Experimental procedures were in agreement with the Animal Research Ethics Committee of the Liaoning Provincial Education Centre for Ecology and Environment, Liaoning University (No. 2013003).

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Authors’ Affiliations

(1)
Provincial Key Laboratory of Animal Resource and Epidemic Disease Prevention, College of Life Sciences, Liaoning University, Shenyang, People’s Republic of China
(2)
Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing, People’s Republic of China

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