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Table 1 Characterization of 24 microsatellite markers from the Emei Shan Liocichla (Liocichla omeiensis)

From: Characterization of novel microsatellite markers of the Emei Shan Liocichla using restriction site-associated DNA sequencing

Locus

Size (bp)

Temperature (°C)

Repeat motif

Primer sequence (5ʹ–3ʹ)

N a

H o

H e

PIC

F IS

r

Chromosome location

LO125

143

61

(ATCT)4

F: AAAAGGTATCATGCCTCTAAGTGG

R: GCAAAAATGGGCTGGGTTAGA

9

0.8824

0.8288

0.7970

−0.0653

0.0000

3

LO131

136

56

(CAGA)4

F: CCTCAGCACAGAACTGGACCTT

R: AAATCGCAGCCTGAATCTCC

4

0.5294

0.5962

0.5320*

0.1130

0.0517

3

LO141

166

58

(AGAT)4

F: CCTCTTAGAAGGCTGCTGTCC

R: CCCTGGTCTTTTCATCTATGTAGTT

6

0.8235

0.7690

0.7230

−0.0717

0.0000

3

LO180

204

55

(AAT)3

F: GGAGAGGAAAATCAGAAAAGGC

R: CTGGGCATCAGTCAGTGGA

10

0.9020

0.8393

0.8110

−0.0755

0.0000

3

LO183

182

57

(GAT)3

F: CATCAACATCATCCAACCCAT

R: AGGCTGGGTCTCAACAATAAAA

5

0.4510

0.5871

0.4930

0.2336

0.1082

3

LO220

168

61

(TTGG)4

F: GATTTGTTTTTCCTTTTGCCTGC

R: GTCGATTGTGGAACTGAGTGAAAA

5

0.5686

0.6327

0.5530

0.1022

0.0395

Z

LO244

145

57

(CAG)3

F: AAAAGAGGGAAACTGGAAGACC

R: ACAGCAGGCAACTGGTATCACT

6

0.7451

0.6544

0.6100

−0.1401

0.0000

Z

LO271

191

58

(AAGA)4

F: TATTGATATTTCCTCCCTCACTGG

R: CAGAATCATTTTCCCCTGGCTA

5

0.7451

0.7177

0.6560

−0.0385

0.0062

Z

LO299

154

58

(ATAG)4

F: TGTAGACGACCATCTTCTGCTGT

R: AGATTGCTGGCTCTTTGGTTATG

6

0.4902

0.7191

0.6700**

0.3205

0.1581

3

LO313

134

57

(TAGG)4

F: CTACGTTACCTTTTTTTCTTCTGC

R: CCCACAAATAAGCAACAAAACA

8

0.5294

0.8464

0.8180**

0.3769

0.1783

2

LO321

160

58

(TTCTA)5

F: GTCAGCCCTGAAGTCGACAAGT

R: GGATTCTTCAGTTGAGGCTGTATTC

6

0.8431

0.7569

0.7090

−0.1152

0.0000

1

LO348

168

61

(GTTG)4

F: GGCACTGGAAAACAGAGGCA

R: GCCTGCCCCTGACATCTGTAT

5

0.7647

0.6564

0.6080

−0.1670

0.0000

3

LO403

162

56

(TGA)3

F: GTGCTTTTTAGGCAGATCTCTCA

R: AAGAACAGGACCCCAGAAAAA

6

0.5686

0.5844

0.5470

0.0272

0.0053

12

LO437

174

57

(AAATA)5

F: CTCTTCACCCACTTCACATCTCTT

R: GAAAAAAATGATGGACAGAAAAGC

10

0.7843

0.7717

0.7440

−0.0165

0.0016

11

LO438

177

57

(TGC)3

F: AGGCTGCCAGGATGTTGCT

R: CAGAGAAGAAGAAAGGGAGGTC

5

0.7451

0.6851

0.6230

−0.0885

0.0000

1

LO445

180

59

(TTAG)4

F: AGATGCAGAAGATTTAGGAGTGTGG

R: TGCCCCCAGCACCTGACTA

6

0.6667

0.7593

0.7110

0.1230

0.0573

8

LO464

217

55

(GCA)3

F: AAAGGGGTGGAGGAAGATAACT

R: TATGCTCATTTTGCTGTTTGC

4

0.7059

0.7173

0.6620

0.0161

0.0023

20

LO514

195

58

(GAT)3

F: TGGGGAGGTCAAGTTGTTCTG

R: CAAAAATTATGTGGACCGATGC

8

0.5098

0.7760

0.7350**

0.3453

0.1666

5

LO520

196

58

(TTTG)4

F: CTGGTGGCTACAGAAGGTGCTC

R: GCACTGGTTTAGTCTCTCCTTGGTA

5

0.3922

0.4628

0.4280

0.1540

0.0754

NA

LO55

153

61

(TGTT)4

F: TGGTGGGGATTTTTTGGTTG

R: CACAGTGCTTCAAGTTGCTCTCTC

5

0.4118

0.6937

0.6390**

0.4088

0.1865

14

LO566

201

56

(CTG)3

F: AAAATCCTGCCCGACTACGA

R: GCTTTATCTCCTCAGCATCTCCT

6

0.6863

0.7556

0.7070

0.0926

0.0413

9

LO579

135

56

(AAAC)4

F: ACAAAATGTAATCAGTTGAAGTCCA

R: GGCAAAAGCATTCTGAAAAGGA

5

0.6078

0.6492

0.5890

0.0643

0.0182

3

LO61

156

53

(TCTA)4

F: GCATATACTTGTACCATGGAAAAC

R: ACCACACAAACTCCAGAACACT

5

0.8431

0.7290

0.6730

−0.1584

0.0000

1A

LO63

186

56

(GGAT)4

F: GCAATGCAACTCCAAAACTCAG

R: CCAGGAAGCAGAAGGACAAAGT

6

0.6863

0.7269

0.6730

0.0564

0.0210

1A

  1. N a, number of alleles at each locus; H o, observed heterozygosity; H e, expected heterozygosity; PIC, polymorphic information content; F IS, inbreeding coefficient; r, frequency of null allele
  2. Asterisk means significantly different after Bonferroni correction for HWE (where * denotes deviation with Bonferroni correction p < 0.05 and ** denotes deviation with Bonferroni correction p < 0.0025)
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