Table 2 Characteristics of three multiplex sets used in genotyping of the Common Pheasant (Phasianus colchicus)
Multiplex sex | Locus | Fluorescent dye | Allele range (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
1 | PC4 | FAM | 87–94 | 56 |
PC8 | ROX | 180–202 |  | |
PC1 | FAM | 201–205 |  | |
PC10 | HEX | 207–241 |  | |
PC9 | FAM | 237–270 |  | |
2 | PC6 | FAM | 84–101 | 57 |
PC7 | ROX | 89–93 |  | |
PC3 | FAM | 152–167 |  | |
PC2 | FAM | 227–240 |  | |
PC5 | FAM | 284–286 |  | |
3 | MCW 127 | ROX | 209–240 | 52 |
MCW 151 | HEX | 251–269 |  | |
MCW 97 | FAM | 266–292 |  |