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Table 1 Degrees of freedom, F-values and significance levels denoting the effect of time since dilution and until analysis, temperature and pH on the sperm motility parameters

From: Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus

Dependent variables Independent variables
Time since dilution and until analysis Temperature pH levels
F df p value F df p value F df p value
Rapid progressive motility 23.902.16, 15.10 < 0.001 2.242, 13 0.15 12.384, 39 < 0.001
Slow progressive motility 2.061.85, 12.95 0.17 4.562, 13 0.03 0.584, 39 0.68
Non-progressive motility 1.017, 49 0.44 1.462, 13 0.27 0.464, 39 0.76
Immotility 10.857, 49 < 0.001 6.842, 13 0.01 6.274, 39 0.001
Progressive motility 20.192.05, 14.32 < 0.001 4.282, 13 0.04 15.264, 39 < 0.001
Sperm velocity 11.657, 49 < 0.001 0.972, 13 0.41 11.224, 39 < 0.001
LIN 2.392.19, 15.35 0.12 0.232, 13 0.80 10.354, 39 < 0.001
WOB 3.082.34, 16.40 0.07 0.212, 13 0.81 10.494, 39 < 0.001
STR 0.392.82, 19.75 0.75 0.472, 13 0.64 7.294, 39 < 0.001
  1. The details of the building of general linear models are shown in Additional file 2: Tables S1‒S3
  2. LIN linearity, WOB wobble, STR straightness
  3. Italic values are significant in the analysis