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Table 1 Degrees of freedom, F-values and significance levels denoting the effect of time since dilution and until analysis, temperature and pH on the sperm motility parameters

From: Optimal analysis conditions for sperm motility parameters with a CASA system in a passerine bird, Passer montanus

Dependent variables

Independent variables

Time since dilution and until analysis

Temperature

pH levels

F df

p value

F df

p value

F df

p value

Rapid progressive motility

23.902.16, 15.10

< 0.001

2.242, 13

0.15

12.384, 39

< 0.001

Slow progressive motility

2.061.85, 12.95

0.17

4.562, 13

0.03

0.584, 39

0.68

Non-progressive motility

1.017, 49

0.44

1.462, 13

0.27

0.464, 39

0.76

Immotility

10.857, 49

< 0.001

6.842, 13

0.01

6.274, 39

0.001

Progressive motility

20.192.05, 14.32

< 0.001

4.282, 13

0.04

15.264, 39

< 0.001

Sperm velocity

11.657, 49

< 0.001

0.972, 13

0.41

11.224, 39

< 0.001

LIN

2.392.19, 15.35

0.12

0.232, 13

0.80

10.354, 39

< 0.001

WOB

3.082.34, 16.40

0.07

0.212, 13

0.81

10.494, 39

< 0.001

STR

0.392.82, 19.75

0.75

0.472, 13

0.64

7.294, 39

< 0.001

  1. The details of the building of general linear models are shown in Additional file 2: Tables S1‒S3
  2. LIN linearity, WOB wobble, STR straightness
  3. Italic values are significant in the analysis