Table 4 PCR success rate, frequency of allelic dropout (ADO) and false alleles (FA) for microsatellite genotyping

From: Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

	Ae36	Ae01	Ae47	Ae44	Ae37	Ae50	Ae05	Ae51	Ae52
Blood (n = 16)
PCR number (N)	48	48	48	48	48	48	48	48	48
PCR success rate (%)	100	100	100	100	100	100	100	100	100
Heterozygotes (H _z)	42	36	39	30	33	39	33	33	45
ADO rate (%)	0	0	0	0	0	0	0	0	0
FA rate (%)	0	0	0	0	0	0	0	0	0
Plucked feathers (n = 31)
PCR number (N)	93	93	94	93	93	93	93	93	93
PCR success rate (%)	100	100	98.94	100	100	100	100	100	100
Heterozygotes (H _z)	72	42	75	81	66	75	63	66	81
ADO rate (%)	0	0	2.67	0	0	0	0	0	0
FA rate (%)	0	0	0	0	0	1.08	0	0	0
Buccal swabs (n = 13)
PCR number (N)	39	39	39	39	39	39	39	39	39
PCR success rate (%)	100	100	100	100	100	100	100	100	100
Heterozygotes (H _z)	33	30	27	30	36	27	30	15	36
ADO rate (%)	0	0	0	0	0	0	0	0	0
FA rate (%)	0	0	0	0	0	0	0	0	0
Eggshell swabs (n = 55)
PCR number (N)	165	167	166	165	166	166	165	166	167
PCR success rate (%)	100	98.80	99.40	100	99.40	99.40	100	99.40	98.80
Heterozygotes (H _z)	144	141	138	99	120	138	117	114	165
ADO rate (%)	0	2.84	1.45	0	1.67	1.45	0	1.75	2.42
FA rate (%)	0	0	0	3.03	0	0	0	0	0
Shed feathers (n = 15)
PCR number (N)	45	45	45	47	47	47	46	46	49
PCR success rate (%)	100	100	100	95.74	95.74	95.74	97.83	97.83	91.84
Heterozygotes (H _z)	42	33	42	42	42	42	33	30	42
ADO rate (%)	0	0	4.76	11.90	9.52	9.52	9.09	6.67	19.05
FA rate (%)	2.22	2.22	0	2.22	0	0	2.22	0	4.44
Feces (n = 11)
PCR number (N)	35	34	35	35	35	35	36	35	35
PCR success rate (%)	94.29	97.06	94.29	94.29	94.29	94.29	91.67	94.29	94.29
Heterozygotes (H _z)	30	30	33	27	27	33	21	30	24
ADO rate (%)	13.33	6.67	12.12	14.81	14.81	12.12	38.10	16.67	29.17
FA rate (%)	9.09	15.15	6.06	9.09	6.06	12.12	6.06	9.09	9.09

Based on three independent PCR replicates of each locus. DNA samples that failed to amplify mtDNA and succeeded in amplifying less than five loci were removed from the analysis. The variable n indicates sample size and N the number of PCR trials. H _z represents heterozygote genotypes used for estimating error rates

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