| |
Ae36
|
Ae01
|
Ae47
|
Ae44
|
Ae37
|
Ae50
|
Ae05
|
Ae51
|
Ae52
|
|---|
|
Blood (n = 16)
|
|
PCR number (N)
|
48
|
48
|
48
|
48
|
48
|
48
|
48
|
48
|
48
|
|
PCR success rate (%)
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
|
Heterozygotes (H
z)
|
42
|
36
|
39
|
30
|
33
|
39
|
33
|
33
|
45
|
|
ADO rate (%)
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
FA rate (%)
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
Plucked feathers (n = 31)
|
|
PCR number (N)
|
93
|
93
|
94
|
93
|
93
|
93
|
93
|
93
|
93
|
|
PCR success rate (%)
|
100
|
100
|
98.94
|
100
|
100
|
100
|
100
|
100
|
100
|
|
Heterozygotes (H
z)
|
72
|
42
|
75
|
81
|
66
|
75
|
63
|
66
|
81
|
|
ADO rate (%)
|
0
|
0
|
2.67
|
0
|
0
|
0
|
0
|
0
|
0
|
|
FA rate (%)
|
0
|
0
|
0
|
0
|
0
|
1.08
|
0
|
0
|
0
|
|
Buccal swabs (n = 13)
|
|
PCR number (N)
|
39
|
39
|
39
|
39
|
39
|
39
|
39
|
39
|
39
|
|
PCR success rate (%)
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
100
|
|
Heterozygotes (H
z)
|
33
|
30
|
27
|
30
|
36
|
27
|
30
|
15
|
36
|
|
ADO rate (%)
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
FA rate (%)
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
0
|
|
Eggshell swabs (n = 55)
|
|
PCR number (N)
|
165
|
167
|
166
|
165
|
166
|
166
|
165
|
166
|
167
|
|
PCR success rate (%)
|
100
|
98.80
|
99.40
|
100
|
99.40
|
99.40
|
100
|
99.40
|
98.80
|
|
Heterozygotes (H
z)
|
144
|
141
|
138
|
99
|
120
|
138
|
117
|
114
|
165
|
|
ADO rate (%)
|
0
|
2.84
|
1.45
|
0
|
1.67
|
1.45
|
0
|
1.75
|
2.42
|
|
FA rate (%)
|
0
|
0
|
0
|
3.03
|
0
|
0
|
0
|
0
|
0
|
|
Shed feathers (n = 15)
|
|
PCR number (N)
|
45
|
45
|
45
|
47
|
47
|
47
|
46
|
46
|
49
|
|
PCR success rate (%)
|
100
|
100
|
100
|
95.74
|
95.74
|
95.74
|
97.83
|
97.83
|
91.84
|
|
Heterozygotes (H
z)
|
42
|
33
|
42
|
42
|
42
|
42
|
33
|
30
|
42
|
|
ADO rate (%)
|
0
|
0
|
4.76
|
11.90
|
9.52
|
9.52
|
9.09
|
6.67
|
19.05
|
|
FA rate (%)
|
2.22
|
2.22
|
0
|
2.22
|
0
|
0
|
2.22
|
0
|
4.44
|
|
Feces (n = 11)
|
|
PCR number (N)
|
35
|
34
|
35
|
35
|
35
|
35
|
36
|
35
|
35
|
|
PCR success rate (%)
|
94.29
|
97.06
|
94.29
|
94.29
|
94.29
|
94.29
|
91.67
|
94.29
|
94.29
|
|
Heterozygotes (H
z)
|
30
|
30
|
33
|
27
|
27
|
33
|
21
|
30
|
24
|
|
ADO rate (%)
|
13.33
|
6.67
|
12.12
|
14.81
|
14.81
|
12.12
|
38.10
|
16.67
|
29.17
|
|
FA rate (%)
|
9.09
|
15.15
|
6.06
|
9.09
|
6.06
|
12.12
|
6.06
|
9.09
|
9.09
|
- Based on three independent PCR replicates of each locus. DNA samples that failed to amplify mtDNA and succeeded in amplifying less than five loci were removed from the analysis. The variable n indicates sample size and N the number of PCR trials. H
z represents heterozygote genotypes used for estimating error rates
