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Table 4 PCR success rate, frequency of allelic dropout (ADO) and false alleles (FA) for microsatellite genotyping

From: Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

  Ae36 Ae01 Ae47 Ae44 Ae37 Ae50 Ae05 Ae51 Ae52
Blood (n = 16)
 PCR number (N) 48 48 48 48 48 48 48 48 48
 PCR success rate (%) 100 100 100 100 100 100 100 100 100
 Heterozygotes (H z) 42 36 39 30 33 39 33 33 45
 ADO rate (%) 0 0 0 0 0 0 0 0 0
 FA rate (%) 0 0 0 0 0 0 0 0 0
Plucked feathers (n = 31)
 PCR number (N) 93 93 94 93 93 93 93 93 93
 PCR success rate (%) 100 100 98.94 100 100 100 100 100 100
 Heterozygotes (H z) 72 42 75 81 66 75 63 66 81
 ADO rate (%) 0 0 2.67 0 0 0 0 0 0
 FA rate (%) 0 0 0 0 0 1.08 0 0 0
Buccal swabs (n = 13)
 PCR number (N) 39 39 39 39 39 39 39 39 39
 PCR success rate (%) 100 100 100 100 100 100 100 100 100
 Heterozygotes (H z) 33 30 27 30 36 27 30 15 36
 ADO rate (%) 0 0 0 0 0 0 0 0 0
 FA rate (%) 0 0 0 0 0 0 0 0 0
Eggshell swabs (n = 55)
 PCR number (N) 165 167 166 165 166 166 165 166 167
 PCR success rate (%) 100 98.80 99.40 100 99.40 99.40 100 99.40 98.80
 Heterozygotes (H z) 144 141 138 99 120 138 117 114 165
 ADO rate (%) 0 2.84 1.45 0 1.67 1.45 0 1.75 2.42
 FA rate (%) 0 0 0 3.03 0 0 0 0 0
Shed feathers (n = 15)
 PCR number (N) 45 45 45 47 47 47 46 46 49
 PCR success rate (%) 100 100 100 95.74 95.74 95.74 97.83 97.83 91.84
 Heterozygotes (H z) 42 33 42 42 42 42 33 30 42
 ADO rate (%) 0 0 4.76 11.90 9.52 9.52 9.09 6.67 19.05
 FA rate (%) 2.22 2.22 0 2.22 0 0 2.22 0 4.44
Feces (n = 11)
 PCR number (N) 35 34 35 35 35 35 36 35 35
 PCR success rate (%) 94.29 97.06 94.29 94.29 94.29 94.29 91.67 94.29 94.29
 Heterozygotes (H z) 30 30 33 27 27 33 21 30 24
 ADO rate (%) 13.33 6.67 12.12 14.81 14.81 12.12 38.10 16.67 29.17
 FA rate (%) 9.09 15.15 6.06 9.09 6.06 12.12 6.06 9.09 9.09
  1. Based on three independent PCR replicates of each locus. DNA samples that failed to amplify mtDNA and succeeded in amplifying less than five loci were removed from the analysis. The variable n indicates sample size and N the number of PCR trials. H z represents heterozygote genotypes used for estimating error rates