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Table 3 Characterization of nine polymorphic microsatellite loci

From: Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

Locus

Size range (bp)

Dye

Annealing temperature (°C)

N A

n

H 0

H e

PIC

HWE

Multiplex 1

 Ae 36

94–114

HEX

62

6

16

0.875

0.813

0.755

0.875

NS

 Ae 01

143–159

TAMRA

62

5

16

0.750

0.685

0.609

0.900

NS

 Ae 47

171–195

FAM

62

7

16

0.813

0.843

0.791

0.344

NS

Multiplex 2

 Ae 44

110–122

HEX

62

4

16

0.625

0.673

0.586

0.868

NS

 Ae 37

165–201

TAMRA

62

7

16

0.688

0.714

0.660

0.447

NS

 Ae 50

211–259

FAM

62

7

16

0.813

0.843

0.791

0.344

NS

Multiplex 3

 Ae 05

165–181

TAMRA

58

5

16

0.688

0.772

0.704

0.070

NS

 Ae 51

110–142

HEX

58

5

16

0.688

0.692

0.626

0.447

NS

 Ae 52

233–403

FAM

58

16

16

0.938

0.954

0.919

0.284

NS

  1. The range of allele sizes is given in base pairs (bp); optimal annealing temperature for amplification (°C)
  2. N A number of observed alleles, n sample size, H 0 observed heterozygosity, H e expected heterozygosity, PIC polymorphism information content, HWE Hardy–Weinberg equilibrium test, NS nonsignificant