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Table 3 Characterization of nine polymorphic microsatellite loci

From: Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

Locus Size range (bp) Dye Annealing temperature (°C) N A n H 0 H e PIC HWE
Multiplex 1
 Ae 36 94–114 HEX 62 6 16 0.875 0.813 0.755 0.875 NS
 Ae 01 143–159 TAMRA 62 5 16 0.750 0.685 0.609 0.900 NS
 Ae 47 171–195 FAM 62 7 16 0.813 0.843 0.791 0.344 NS
Multiplex 2
 Ae 44 110–122 HEX 62 4 16 0.625 0.673 0.586 0.868 NS
 Ae 37 165–201 TAMRA 62 7 16 0.688 0.714 0.660 0.447 NS
 Ae 50 211–259 FAM 62 7 16 0.813 0.843 0.791 0.344 NS
Multiplex 3
 Ae 05 165–181 TAMRA 58 5 16 0.688 0.772 0.704 0.070 NS
 Ae 51 110–142 HEX 58 5 16 0.688 0.692 0.626 0.447 NS
 Ae 52 233–403 FAM 58 16 16 0.938 0.954 0.919 0.284 NS
  1. The range of allele sizes is given in base pairs (bp); optimal annealing temperature for amplification (°C)
  2. N A number of observed alleles, n sample size, H 0 observed heterozygosity, H e expected heterozygosity, PIC polymorphism information content, HWE Hardy–Weinberg equilibrium test, NS nonsignificant