|
Locus
|
Size range (bp)
|
Dye
|
Annealing temperature (°C)
|
N
A
|
n
|
H
0
|
H
e
|
PIC
|
HWE
|
|---|
|
Multiplex 1
|
|
Ae 36
|
94–114
|
HEX
|
62
|
6
|
16
|
0.875
|
0.813
|
0.755
|
0.875
|
NS
|
|
Ae 01
|
143–159
|
TAMRA
|
62
|
5
|
16
|
0.750
|
0.685
|
0.609
|
0.900
|
NS
|
|
Ae 47
|
171–195
|
FAM
|
62
|
7
|
16
|
0.813
|
0.843
|
0.791
|
0.344
|
NS
|
|
Multiplex 2
|
|
Ae 44
|
110–122
|
HEX
|
62
|
4
|
16
|
0.625
|
0.673
|
0.586
|
0.868
|
NS
|
|
Ae 37
|
165–201
|
TAMRA
|
62
|
7
|
16
|
0.688
|
0.714
|
0.660
|
0.447
|
NS
|
|
Ae 50
|
211–259
|
FAM
|
62
|
7
|
16
|
0.813
|
0.843
|
0.791
|
0.344
|
NS
|
|
Multiplex 3
|
|
Ae 05
|
165–181
|
TAMRA
|
58
|
5
|
16
|
0.688
|
0.772
|
0.704
|
0.070
|
NS
|
|
Ae 51
|
110–142
|
HEX
|
58
|
5
|
16
|
0.688
|
0.692
|
0.626
|
0.447
|
NS
|
|
Ae 52
|
233–403
|
FAM
|
58
|
16
|
16
|
0.938
|
0.954
|
0.919
|
0.284
|
NS
|
- The range of allele sizes is given in base pairs (bp); optimal annealing temperature for amplification (°C)
-
N
A number of observed alleles, n sample size, H
0 observed heterozygosity, H
e expected heterozygosity, PIC polymorphism information content, HWE Hardy–Weinberg equilibrium test, NS nonsignificant
