Noninvasive and nondestructive sampling for avian microsatellite genotyping: a case study on the vulnerable Chinese Egret (Egretta eulophotes)

Table 1 Number of DNA samples collected and successfully amplified with various primers

Sample type	Invasiveness category	N _a	N _b	N _c	N _d
Blood	Invasive	16	16	16	16
Plucked feathers	Nondestructive	31	31	31	31
Buccal swabs	Nondestructive	13	13	13	13
Eggshell swabs	Noninvasive	64	64	62	55^a
Shed feathers	Noninvasive	16	15	15	15
Feces	Noninvasive	19	13	11	11
Total		159	152	148	141

N _a number of samples collected in the field, N _b number of samples that successfully amplified mtDNA, N _c number of samples successfully amplified with the CHD gene, N _d number of samples that successfully amplified more than 5 microsatellite loci
^aFive eggshell swabs with DNA contamination were also eliminated

ISSN: 2053-7166