Table 1 Characteristics of 13 microsatellites in a sample set of 90 individuals of the Common Pheasant (Phasianus colchicus)
Locus | Primer sequence | Repeat motif | Annealing temperature (°C) | Size range (bp) | N A | H O | H E | PIC |
|---|---|---|---|---|---|---|---|---|
PC 1 | F: AGCACATCACAGTGCTTTGAGC | (AT)5 | 58 | 201–205 | 4.2 | 0.440 | 0.680 | 0.635 |
R: TTTGCTCAGGAAAAGAAAATAAAGACA | ||||||||
PC 2 | F: AAAAAGCTCATTTGCTGTGGAA | (AT)10 | 56 | 227–240 | 3.4 | 0.460 | 0.707 | 0.672 |
R: TCTTTGTCTTCACCCTCATGGA | ||||||||
PC 3 | F: GAGGGTAGAGAGAAACAGGTGTTGA | (CA)7 | 57 | 152–167 | 7.6 | 0.620 | 0.886 | 0.871 |
R: GAGGTAATCTCTCACTGCTGATTGG | ||||||||
PC 4 | F: TTCCAAAAGCATATCCCAGAGC | (AC)7 | 58 | 87–94 | 1.6 | 0.110 | 0.274 | 0.254 |
R: TAAGATAGCCCATCCTTTGGGG | ||||||||
PC 5 | F: TGACCACTACAGTTTCCCATTCTTC | (CT)8 | 57 | 284–286 | 4.4 | 0.540 | 0.801 | 0.777 |
R: AGATCTTCAGTAGCTCTTGGAACACA | ||||||||
PC 6 | F: ACGGTCAGTAAGCATGTACCCC | (TG)10 | 57 | 84–101 | 6 | 0.500 | 0.763 | 0.701 |
R: AGCAGTCAATGGAGAGCAGGTT | ||||||||
PC 7 | F: GGCTGTCCTTTTAGCTACAGCAG | (AT)13 | 57 | 89–93 | 7.8 | 0.830 | 0.905 | 0.893 |
R: CATCATCAAGAAGCATTGCAAAA | ||||||||
PC 8 | F: GACCTCTGTCATTGGTTTTGGA | (TG)14 | 56 | 180–202 | 1.3 | 0.030 | 0.049 | 0.048 |
R: TATGATTGTGAACAGCTGCCAA | ||||||||
PC 9 | F: AATGGGAACTTTTTCAGGGACAA | (AC)14 | 58 | 237–270 | 2.8 | 0.310 | 0.559 | 0.504 |
R: TTTGAAGTTGGTGGGACTCCAT | ||||||||
PC 10 | F: GCTGCAAATCTCCTTAGCTCCA | (TG)16 | 58 | 207–242 | 11 | 0.579 | 0.905 | 0.869 |
R: GGAGCAACAGTGGGAGAAGAAA | ||||||||
MCW 97 | F: GGAGAGCATCTGCCTTCCTAG | (AT)8C(AT)6C(AT)10C | 50 | 266–292 | 2.1 | 0.980 | 0.512 | 0.389 |
R: TGGTCTTCCAGTCTATGGTAG | ||||||||
MCW 127 | F: TGCAATAAGAGAAGGTAAGGTC | (TA)7 | 50 | 209–240 | 2.0 | 0.290 | 0.478 | 0.397 |
R: GAGTTCAGCAGGAATGGGATG | ||||||||
MCW 151 | F: CATGCTGTGATACTACAATTCC | (CA)8 | 50 | 251–269 | 2.8 | 0.370 | 0.618 | 0.588 |
R: AACATCCTGGAGTTTGGGAAG |